Title: Gnotobiotic Mouse Technology: An Illustrated Guide
Authors: Chriss J. Vowles, Natalie E. Anderson, and Kathryn A. Eaton
Publisher: CRC Press / Taylor & Francis
Publication Year: 2016
ISBN-13: 978-1-4987-3633-6
Gnotobiotic Mouse Technology: An Illustrated Guide delivers a comprehensive, highly visual manual detailing the establishment, maintenance, and experimental manipulation of germ-free and gnotobiotic mouse models. Authored by experienced research specialists Chriss J. Vowles, Natalie E. Anderson, and Kathryn A. Eaton, this practical reference bridges foundational housing techniques with advanced microbiological isolation protocols. It serves as an essential, indispensable resource for animal facility managers, biomedical researchers, and laboratory technicians.
Key Topics Covered:
- Isolation Housing & Equipment: Step-by-step instructions for operating flexible film isolators, microisolator cages, and sterilizing pass-through ports.
- Sterilization & Aseptic Technique: Protocols for chemical disinfection, autoclaving supplies, and maintaining strict microbiological barrier integrity.
- Microbial Colonization & Monitoring: Procedures for introducing defined microbiota, performing contamination checks, and conducting routine quality control cultures.
- Colony Management & Husbandry: Best practices for breeding, feeding, handling, and tracking germ-free and gnotobiotic rodent populations in research environments.
Reviews
There are no reviews yet.