Title: Laboratory Handbook on Bovine Mastitis (Third Edition)
Editors: John R. Middleton, Lawrence K. Fox, and Gina Pighetti
Publisher: National Mastitis Council, Inc.
Publication Year: 2017
ISBN-13: 978-0-9664536-1-8
Laboratory Handbook on Bovine Mastitis, Third Edition offers an authoritative, international reference for diagnosing, monitoring, and controlling intramammary infections in dairy cattle. Published by the National Mastitis Council and edited by leading veterinary experts John R. Middleton, Lawrence K. Fox, and Gina Pighetti, this fully updated edition combines trusted microbiological techniques with modern molecular diagnostics and on-farm culture strategies. It serves as an essential, indispensable resource for veterinarians, diagnostic laboratory personnel, dairy researchers, and industry specialists.
Key Topics Covered:
- Standard Microbial Culture: Proven laboratory protocols for collecting, culturing, and identifying common and emerging mastitis-causing pathogens.
- Molecular Diagnostic Techniques: Up-to-date evaluations of PCR and molecular assays, focusing on accuracy, specificity, sensitivity, and turnaround times.
- Specialized Pathogen Identification: Dedicated frameworks for isolating challenging organisms, including specialized chapters on Mycoplasma species.
- On-Farm Culture & Control Programs: Practical guidelines for implementing on-farm diagnostic testing systems and mastitis management strategies.






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